Thursday, May 29, 2025

Do My Data Need to Be Normally Distributed to Use ANOVA?

This is one of the most frequent questions asked by those starting to analyze data:


“Do my data need to be normally distributed?”

Let’s clarify:

To apply analysis of variance (ANOVA), it is not necessary for the data themselves to follow a normal distribution.

What is required is that the residuals (or errors) from the ANOVA model are approximately normally distributed.

πŸ” What are residuals?

A residual is the difference between an observed value and the mean of the group it belongs to.

In a one-way ANOVA, the residual is calculated as:

                           Residual = observed value – group mean

πŸ“Š Example:

Imagine the data shown in Table 1. The group means are listed at the bottom of the table.

                                                         Table 1– Data from a trial

Based on these values, we calculate the residuals by subtracting the group mean from each data point.
                                                         Table 2 – Residuals

πŸ“ˆ Why analyze residuals?

The study of residuals — called residual analysis — is crucial because ANOVA assumes that residuals are normally distributed.

That’s why we always need to analyze the residuals when using ANOVA.

πŸ§ͺ How do we analyze residuals?

A good practice is to examine residuals graphically and use statistical tests to verify that ANOVA assumptions are met. Figure 1 shows the histogram from Table 2 residuals.

                                          Figure 1. Histogram of residuals

Even if it’s not a “perfect normal distribution,” note the symmetry — this is a good sign. ANOVA is robust to minor violations of normality, especially when residuals are approximately symmetric. Figure 2 shows the boxplot of the residuals. 

Figure 2. Boxplot

Symmetry and absence of outliers strengthen the case that ANOVA assumptions are satisfied. The Q-Q plot (quantile-quantile) compares observed residuals to what would be expected under normality. If points align along a 45° line, that’s a good sign. The P-P plot is another visual tool to assess normality.

                                                 Figure 3 – Q-Q plot of the residuals

                                       

πŸ“Œ Descriptive statistics of residuals

  Some summary measures help evaluate the distribution:

Mean and median: If equal or close → symmetry
Skewness coefficient: Close to zero → good
Kurtosis: Negative values suggest a flatter distribution, which is not necessarily problematic

                     Table 3 – Descriptive statistics of residuals

                                    

In our example:
Mean = 0
Median = 0
· Skewness = 0 (symmetric)
· Slightly negative kurtosis (light tails), but still acceptable

Statistical tests of normality

    Normality tests provide objective checks. The most common ones include:
              
Shapiro-Wilk
             
Kolmogorov-Smirnov

In our example, the Kolmogorov-Smirnov test was performed in SPSS and resulted in p = 0.200.

That means there is no evidence to reject normality of the residuals.

⚠️ A note on sample size:

Small samples: less power to detect non-normality.
Large samples: may detect minor deviations that don't impact ANOVA results.

🧠 Final thoughts:

When group sizes are equal and fixed factors are used, ANOVA remains reliable despite slight violations of normality.
Problems arise mainly with high skewness or very different group variances.

πŸ’‘ Important takeaway:

Raw data are usually not normally distributed, because they come from distinct groups expected to have different means.

What matters is:

Whether the residuals follow a normal distribution, or
Even better, whether each group’s data is normally distributed.

πŸ“š References:

1. Ghasemi, A. & Zahediasl, S. (2012). Normality Tests for Statistical Analysis: A Guide for Non-Statisticians. Int J Endocrinol Metab. 10(2): 486–489.
2. ScheffΓ©, H. (1959). The Analysis of Variance. Wiley.




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